Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing
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چکیده
منابع مشابه
Zebrafish keratocyte explants to study collective cell migration and reepithelialization in cutaneous wound healing.
Due to their unique motile properties, fish keratocytes dissociated from explant cultures have long been used to study the mechanisms of single cell migration. However, when explants are established, these cells also move collectively, maintaining many of the features which make individual keratocytes an attractive model to study migration: rapid rates of motility, extensive actin-rich lamellae...
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During embryonic morphogenesis, wound repair and cancer invasion, cells often migrate collectively via tight cell-cell junctions, a process named collective migration. During such migration, cells move as coherent groups, large cell sheets, strands or tubes rather than individually. One unexpected finding regarding collective cell migration is that being a "multicellular structure" enables cell...
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Wound healing is a physiological process of repair of a tissue that has been structurally damaged. The most common wounds disrupt one of the epithelial tissues that protect the internal and external surfaces of the body and act as barriers against invasion by microorganisms. Such tissues include skin, corneal tissue, and the epithelial lining of the digestive tract (including the mouth and esop...
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Collective cell migration plays essential roles in a wide spectrum of biological processes, such as embryogenesis, tissue regeneration, and cancer metastasis. Numerous wound-healing assays based on mechanical, chemical, optical, and electrical approaches have been developed to create model "wounds" in cell monolayers to study the collective cell migration processes. These approaches can result ...
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Collective cell migration plays an important role during wound healing and embryo development. Although the exact mechanisms that coordinate such migration are still unknown, experimental studies of moving cell layers have shown that the primary interactions governing the motion of the layer are the force of lamellipodia, the adhesion of cells to the substrate, and the adhesion of cells to each...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2015
ISSN: 1940-087X
DOI: 10.3791/52489